eI4EBP2 Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3839R-Cy5 |
| Product Name | eI4EBP2 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 4E-BP2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1979 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Regulates e4E activity by preventing its assembly into the e4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway.Full=Eukaryotic translation initiation factor 4E-binding protein 2; 4E-BP2; e4E-binding protein 2; 4EBP2_HUMAN. |
| Background | 4E-BP2; 4EBP2; eIF4E binding protein 2; eIF4E-binding protein 2; Eif4ebp2; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 2; PHASII. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eI4EBP2 Polyclonal Antibody, Cy5 Conjugated (bs-3839R-Cy5) validated for, and at what dilution?
bs-3839R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3839R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eI4EBP2 Polyclonal Antibody, Cy5 Conjugated (bs-3839R-Cy5)?
KLH conjugated synthetic peptide derived from human 4E-BP2
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3839R-Cy5)?
Cy5 on bs-3839R-Cy5: excitation / emission 625,650nm/670nm.