eIF3H Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3842R-BF488-100uL |
| Product Name | eIF3H Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human eIF3H |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8667 |
| Subcellular location | Cytoplasm |
| Synonyms | e3S3 e3 gamma; e3 p40; e3 p40 subunit; e3h; Eukaryotic translation initiation factor 3 subunit 3; MGC102958; E3H_HUMAN. |
| Background | EIF3S3 binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. It associates with the p170 subunit of EIF3. The EIF3 is composed of at least 12 different subunits. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is eIF3H Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3842R-BF488) validated for, and at what dilution?
bs-3842R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3842R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise eIF3H Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3842R-BF488)?
KLH conjugated synthetic peptide derived from human eIF3H
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-3842R-BF488)?
AbBy Fluor-488 on bs-3842R-BF488: excitation / emission 499nm/519nm.