SCAP Polyclonal Antibody, PE Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3862R-PE-100uL |
| Product Name | SCAP Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SCAP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22937 |
| Swiss Prot | Q12770 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | KIAA0199; SCAP; SCAP_HUMAN; SREBF chaperone; SREBF chaperone protein; SREBP cleavage activating protein; SREBP cleavage-activating protein; Sterol regulatory element binding protein cleavage-activating protein; Sterol regulatory element-binding protein cleavage-activating protein. |
| Background | SCAP is an escort protein required for cholestrol and lipid homeostasis. Cholesterol homeostasis in animal cells is achieved by regulated cleavage of SREBPs, membrane-bound transcription factors. SCAP forms a complex with SREBPs in order to release the active domains of SREBPs. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is SCAP Polyclonal Antibody, PE Conjugated (bs-3862R-PE) validated for, and at what dilution?
bs-3862R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3862R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SCAP Polyclonal Antibody, PE Conjugated (bs-3862R-PE)?
KLH conjugated synthetic peptide derived from human SCAP
What are the excitation and emission wavelengths of the PE conjugate (bs-3862R-PE)?
PE on bs-3862R-PE: excitation / emission 496,564nm/578nm.