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GATA1 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-3872R-Biotin-100uL
Product Name GATA1 Polyclonal Antibody, Biotin Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GATA1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 2623
Swiss Prot P15976
Subcellular location Nucleus
Synonyms GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1; Erythroid transcription factor; GATA-binding factor 1; NF-E1 DNA-binding protein; GATA1
Background Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is GATA1 Polyclonal Antibody, Biotin Conjugated (bs-3872R-Biotin) validated for, and at what dilution?

bs-3872R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GATA1 Polyclonal Antibody, Biotin Conjugated (bs-3872R-Biotin) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-3872R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GATA1 Polyclonal Antibody, Biotin Conjugated (bs-3872R-Biotin)?

KLH conjugated synthetic peptide derived from human GATA1