ALOX12/12 Lipoxygenase Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3874R-APC-Cy7-100uL |
| Product Name | ALOX12/12 Lipoxygenase Antibody, APC-Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human 12 Lipoxygenase |
| Immunogen Range | 101-200/663 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 239 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | 12 Lox; 12S lipoxygenase; 12S LOX; Alox 12; Alox12; Alox12L; Alox15; Arachidonate 12 lipoxygenase 12S type; Arachidonate 12 lipoxygenase; Arachidonate 12 lipoxygenase leukocyte type; Arachidonate 12 lipoxygenase, 12S type; Arachidonate 12 lipoxygenase, leukocyte type; EC1.13.11.31; LOG 12; LOG12; Platelet type 12 lipoxygenase; Platelet type lipoxygenase 12; P-12-LOX. |
| Background | 12 Lipoxygenase is a non heme iron containing dioxygenase which stereospecifically incorporates molecular oxygen into cis,cis-1,4-pentadiene containing polyunsaturated fatty acids. Murine leukocyte 12 Lipoxygenase oxidizes arachidonic acid to 12(S)-HpETE and 15(S)-HpETE in a ratio of 3:1. Murine leukocyte 12 Lipoxygenase is a 75 kDa protein which is 58% homologous to murine platelet 12 Lipoxygenase, and 70% homologous to porcine leukocyte 12 Lipoxygenase. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ALOX12/12 Lipoxygenase Antibody, APC-Cy7 Conjugated (bs-3874R-APC-Cy7) validated for, and at what dilution?
bs-3874R-APC-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ALOX12/12 Lipoxygenase Antibody, APC-Cy7 Conjugated (bs-3874R-APC-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow.
How should bs-3874R-APC-Cy7 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ALOX12/12 Lipoxygenase Antibody, APC-Cy7 Conjugated (bs-3874R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human 12 Lipoxygenase Immunogen range: 101-200/663.
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3874R-APC-Cy7)?
APC-Cy7 on bs-3874R-APC-Cy7: excitation / emission 650nm/780nm.