Dio3 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3902R-Cy7-100uL |
| Product Name | Dio3 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Dio3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1735 |
| Subcellular location | Cell membrane |
| Synonyms | Type 3 DI; D3; 5DIII; Deiodinase iodothyronine type 3; Deiodinase iodothyronine type III; Dio 3; DIOIII; MGC124117; MGC124118; TXDI3; Type III 5' deiodinase; Type III iodothyronine deiodinase; IOD3_HUMAN. |
| Background | Dio3 belongs to the iodothyronine deiodinase family. It has an essential role for regulation of thyroid hormone inactivation during embryological development in that it catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. Dio3 may play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones and has been linked to the development of consumptive hypothyroidism in both infants and adults. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Dio3 Polyclonal Antibody, Cy7 Conjugated (bs-3902R-Cy7) validated for, and at what dilution?
bs-3902R-Cy7 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Dio3 Polyclonal Antibody, Cy7 Conjugated (bs-3902R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig.
How should bs-3902R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Dio3 Polyclonal Antibody, Cy7 Conjugated (bs-3902R-Cy7)?
KLH conjugated synthetic peptide derived from human Dio3
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3902R-Cy7)?
Cy7 on bs-3902R-Cy7: excitation / emission 743nm/767nm.