Legumain Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3907R-FITC |
| Product Name | Legumain Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Horse |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Legumain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5641 |
| Swiss Prot | Q99538 |
| Subcellular location | Cytoplasm |
| Synonyms | AEP; LGMN1; PRSC1; Legumain; Asparaginyl endopeptidase; Protease, cysteine 1; LGMN |
| Background | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is Legumain Polyclonal Antibody, FITC Conjugated (bs-3907R-FITC) validated for, and at what dilution?
bs-3907R-FITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Legumain Polyclonal Antibody, FITC Conjugated (bs-3907R-FITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Horse.
How should bs-3907R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Legumain Polyclonal Antibody, FITC Conjugated (bs-3907R-FITC)?
KLH conjugated synthetic peptide derived from human Legumain
What are the excitation and emission wavelengths of the FITC conjugate (bs-3907R-FITC)?
FITC on bs-3907R-FITC: excitation / emission 494nm/518nm.