Megalin Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Megalin Polyclonal Antibody, Unconjugated (bs-3909R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Megalin Polyclonal Antibody, Unconjugated (bs-3909R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Megalin Polyclonal Antibody(bs-3909R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Mouse kidney were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Megalin Polyclonal Antibody(bs-3909R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Horse
Chicken
Rabbit

Overview
Catalog #	bs-3909R
Product Name	Megalin Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Megalin
Immunogen Range	3401-3500/4655
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4036
Swiss Prot	P98164
Subcellular location	Cell membrane
Synonyms	DBS; GP33; Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 33; Megalin; LRP2
Background	Acts together with cubilin to mediate HDL endocytosis (By similarity). May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500

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