ACLY Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3929R-PE-Cy5-100uL |
| Product Name | ACLY Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATP citrate lyase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 47 |
| Subcellular location | Cytoplasm |
| Synonyms | ACL; ATP citrate pro-S lyase; ATP citrate synthase; Citrate cleavage enzyme; EC 2.3.3.8; A730098H14RIK; ACLY; ATP CITRATE LYASE; ATPCL; AW538652; Cce; CITRATE LYASE; CLATP; MGC124629. |
| Background | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is ACLY Polyclonal Antibody, PE-Cy5 Conjugated (bs-3929R-PE-Cy5) validated for, and at what dilution?
bs-3929R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ACLY Polyclonal Antibody, PE-Cy5 Conjugated (bs-3929R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-3929R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ACLY Polyclonal Antibody, PE-Cy5 Conjugated (bs-3929R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human ATP citrate lyase
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3929R-PE-Cy5)?
PE-Cy5 on bs-3929R-PE-Cy5: excitation / emission 488nm/670nm.