GNB2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3942R-Cy3 |
| Product Name | GNB2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human G protein beta 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2783 |
| Subcellular location | Cytoplasm |
| Synonyms | G protein beta 2 subunit; G protein subunit beta 2; G protein, beta 2 subunit; Gnb2l1; guanine nucleotide binding protein G protein, beta polypeptide 2; Guanine nucleotide binding protein beta 2 subunit; Guanine nucleotide binding protein G I G S G T beta 2 subunit 2; Guanine nucleotide binding protein G protein beta polypeptide 2; guanine nucleotide binding protein, beta 2 subunit; RACK1; Receptor for activated C kinase; Receptor of activated protein kinase C 1; signal transducing guanine nucleotide binding regulatory protein beta; Transducin beta chain 2; OTTHUMP00000174601; OTTHUMP00000174602; GBB2_HUMAN. |
| Background | GNB2 belongs to the WD repeat G protein beta family. Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is GNB2 Polyclonal Antibody, Cy3 Conjugated (bs-3942R-Cy3) validated for, and at what dilution?
bs-3942R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does GNB2 Polyclonal Antibody, Cy3 Conjugated (bs-3942R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-3942R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise GNB2 Polyclonal Antibody, Cy3 Conjugated (bs-3942R-Cy3)?
KLH conjugated synthetic peptide derived from human G protein beta 2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3942R-Cy3)?
Cy3 on bs-3942R-Cy3: excitation / emission 512,550nm/570,615nm.