MT-ND5 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3952R-RBITC-100uL |
| Product Name | MT-ND5 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MT-ND5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4540 |
| Swiss Prot | P03915 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | MTND5; NADH-ubiquinone oxidoreductase chain 5; NADH dehydrogenase subunit 5; MT-ND5; NADH5; ND5 |
| Background | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MT-ND5 Polyclonal Antibody, RBITC Conjugated (bs-3952R-RBITC) validated for, and at what dilution?
bs-3952R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MT-ND5 Polyclonal Antibody, RBITC Conjugated (bs-3952R-RBITC) react with?
Tested reactivity: Human, Rat.
How should bs-3952R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MT-ND5 Polyclonal Antibody, RBITC Conjugated (bs-3952R-RBITC)?
KLH conjugated synthetic peptide derived from human MT-ND5
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3952R-RBITC)?
RBITC on bs-3952R-RBITC: excitation / emission 540nm/625nm.