NDUFA10 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3957R-Cy3-100uL |
| Product Name | NDUFA10 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFA10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4705 |
| Swiss Prot | O95299 |
| Subcellular location | Cytoplasm |
| Synonyms | CI-42k; CI-42KD; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial; Complex I-42kD; NADH-ubiquinone oxidoreductase 42 kDa subunit; NDUFA10 |
| Background | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NDUFA10 Polyclonal Antibody, Cy3 Conjugated (bs-3957R-Cy3) validated for, and at what dilution?
bs-3957R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDUFA10 Polyclonal Antibody, Cy3 Conjugated (bs-3957R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-3957R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFA10 Polyclonal Antibody, Cy3 Conjugated (bs-3957R-Cy3)?
KLH conjugated synthetic peptide derived from human NDUFA10
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3957R-Cy3)?
Cy3 on bs-3957R-Cy3: excitation / emission 512,550nm/570,615nm.