NDUFA8 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3958R-Cy7-100uL |
| Product Name | NDUFA8 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFA8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4702 |
| Swiss Prot | P51970 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PGIV; CI-19KD; CI-PGIV; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; Complex I-19kD; Complex I-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit; NDUFA8 |
| Background | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NDUFA8 Polyclonal Antibody, Cy7 Conjugated (bs-3958R-Cy7) validated for, and at what dilution?
bs-3958R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDUFA8 Polyclonal Antibody, Cy7 Conjugated (bs-3958R-Cy7) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse.
How should bs-3958R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFA8 Polyclonal Antibody, Cy7 Conjugated (bs-3958R-Cy7)?
KLH conjugated synthetic peptide derived from human NDUFA8
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3958R-Cy7)?
Cy7 on bs-3958R-Cy7: excitation / emission 743nm/767nm.