NDUFS4 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3961R-BF647-100uL |
| Product Name | NDUFS4 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDUFS4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4724 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | AQDQ; CI 18 kDa; CI AQDQ; Complex I 18 kDa; Complex I AQDQ; mitochondrial respiratory chain complex I 18 KD subunit; NADH dehydrogenase; NADH ubiquinone oxidoreductase 18 kDa subunit; NDUS4_HUMAN. |
| Background | This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NDUFS4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3961R-BF647) validated for, and at what dilution?
bs-3961R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3961R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDUFS4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3961R-BF647)?
KLH conjugated synthetic peptide derived from human NDUFS4
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3961R-BF647)?
AbBy Fluor-647 on bs-3961R-BF647: excitation / emission 650nm/665nm.