PPM2C Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3966R-Cy5.5-100uL |
| Product Name | PPM2C Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Dog |
| Predicted Reactivity | Human, Mouse, Rat, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PPM2C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 54704 |
| Subcellular location | Cytoplasm |
| Synonyms | PDH; PDP 1; PDP; PDP1; PDPC 1; PDPC; Protein phosphatase 2C; protein phosphatase 2C, magnesium dependent, catalytic subunit; Pyruvate dehydrogenase phosphatase, catalytic subunit 1; PDP1_HUMAN. |
| Background | PPM2C is a protein similar to members of the protein phosphatase 2C (PP2C) family. It is a catalytic subunit of the mitochondrial matrix phosphatase and is involved in reactivation of the pyruvate dehydrogenase complex. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PPM2C Polyclonal Antibody, Cy5.5 Conjugated (BS-3966R-CY5-5) validated for, and at what dilution?
BS-3966R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PPM2C Polyclonal Antibody, Cy5.5 Conjugated (BS-3966R-CY5-5) react with?
Tested reactivity: Dog. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat, Cow, Horse, Rabbit.
How should BS-3966R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PPM2C Polyclonal Antibody, Cy5.5 Conjugated (BS-3966R-CY5-5)?
KLH conjugated synthetic peptide derived from human PPM2C
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3966R-CY5-5)?
Cy5.5 on BS-3966R-CY5-5: excitation / emission 675nm/694nm.