AMPK gamma 3 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3968R-PE-Cy5-100uL |
| Product Name | AMPK gamma 3 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AMPK gamma 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 53632 |
| Swiss Prot | Q9UGI9 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | AMPKG3; 5'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3; PRKAG3 |
| Background | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is AMPK gamma 3 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3968R-PE-Cy5) validated for, and at what dilution?
bs-3968R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3968R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AMPK gamma 3 Polyclonal Antibody, PE-Cy5 Conjugated (bs-3968R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human AMPK gamma 3
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3968R-PE-Cy5)?
PE-Cy5 on bs-3968R-PE-Cy5: excitation / emission 488nm/670nm.