SDHA Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3970R-Cy5 |
| Product Name | SDHA Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SDHA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6389 |
| Swiss Prot | P31040 |
| Subcellular location | Cytoplasm |
| Synonyms | FP; PGL5; SDH1; SDH2; SDHF; CMD1GG; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; Flavoprotein subunit of complex II; SDHA |
| Background | Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SDHA Polyclonal Antibody, Cy5 Conjugated (bs-3970R-Cy5) validated for, and at what dilution?
bs-3970R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SDHA Polyclonal Antibody, Cy5 Conjugated (bs-3970R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should bs-3970R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SDHA Polyclonal Antibody, Cy5 Conjugated (bs-3970R-Cy5)?
KLH conjugated synthetic peptide derived from human SDHA
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3970R-Cy5)?
Cy5 on bs-3970R-Cy5: excitation / emission 625,650nm/670nm.