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ENO3 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-3979R-Biotin-100uL
Product Name ENO3 Polyclonal Antibody, Biotin Conjugated
Applications
WB IHC-P IHC-F
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ENO3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C for 12 months.
Target
Gene ID 2027
Swiss Prot P13929
Subcellular location Cytoplasm
Synonyms MSE; GSD13; Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; Skeletal muscle enolase; ENO3
Background ENO3 is also known as beta enolase, one of the three enolase isoenzymes found in mammals. A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, a homodimer, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration. Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy. This results in exercise-induced myalgias, generalized muscle weakness and fatigability.
Application Dilution
WB IHC-P1:200-400
IHC-P
IHC-F

Frequently asked questions

Which applications is ENO3 Polyclonal Antibody, Biotin Conjugated (bs-3979R-Biotin) validated for, and at what dilution?

bs-3979R-Biotin is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ENO3 Polyclonal Antibody, Biotin Conjugated (bs-3979R-Biotin) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Chicken.

How should bs-3979R-Biotin be stored?

Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ENO3 Polyclonal Antibody, Biotin Conjugated (bs-3979R-Biotin)?

KLH conjugated synthetic peptide derived from human ENO3

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