ENO3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3979R |
| Product Name | ENO3 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ENO3 |
| Immunogen Range | 341-434/434 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2027 |
| Swiss Prot | P13929 |
| Subcellular location | Cytoplasm |
| Synonyms | MSE; GSD13; Beta-enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Muscle-specific enolase; Skeletal muscle enolase; ENO3 |
| Background | ENO3 is also known as beta enolase, one of the three enolase isoenzymes found in mammals. A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, a homodimer, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration. Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy. This results in exercise-induced myalgias, generalized muscle weakness and fatigability. |
| Application Dilution | |
| WB | IHC-P1:200-400 |
| IHC-P | |
| IHC-F | |
| IF | |
| Flow-Cyt | |
Frequently asked questions
Which applications is ENO3 Polyclonal Antibody (bs-3979R) validated for, and at what dilution?
bs-3979R is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ENO3 Polyclonal Antibody (bs-3979R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Chicken.
How should bs-3979R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ENO3 Polyclonal Antibody (bs-3979R)?
KLH conjugated synthetic peptide derived from human ENO3 Immunogen range: 341-434/434.