Fibrillarin Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3998R-BF488-100uL |
| Product Name | Fibrillarin Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Fibrillarin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2091 |
| Subcellular location | Nucleus |
| Synonyms | 34 kD nucleolar scleroderma antigen; 34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL_HUMAN; FIB; FIB1; FLRN; Nop1p; RNU3IP1; rRNA 2' O methyltransferase fibrillarin; rRNA 2''-O-methyltransferase fibrillarin; rRNA 2'-O-methyltransferase fibrillarin. |
| Background | Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Fibrillarin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3998R-BF488) validated for, and at what dilution?
bs-3998R-BF488 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Fibrillarin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3998R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig.
How should bs-3998R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fibrillarin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-3998R-BF488)?
KLH conjugated synthetic peptide derived from human Fibrillarin
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-3998R-BF488)?
AbBy Fluor-488 on bs-3998R-BF488: excitation / emission 499nm/519nm.