ATG4C Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4008R-Cy3-100uL |
| Product Name | ATG4C Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATG4C |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84938 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | APG4 autophagy 4 homolog C S. cerevisiae; APG4 autophagy 4 homolog C; APG4 C; APG4-C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C S. cerevisiae; Atg4C; ATG4C_HUMAN; AUT S. cerevisiae like 1, cysteine endopeptidase; AUT like 1, cysteine endopeptidase S. cerevisiae; AUT like 1, cysteine endopeptidase; AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; Cysteine protease ATG4C; EC 3.4.22; FLJ14867; OTTHUMP00000010715. |
| Background | Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ATG4C Polyclonal Antibody, Cy3 Conjugated (bs-4008R-Cy3) validated for, and at what dilution?
bs-4008R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATG4C Polyclonal Antibody, Cy3 Conjugated (bs-4008R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should bs-4008R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATG4C Polyclonal Antibody, Cy3 Conjugated (bs-4008R-Cy3)?
KLH conjugated synthetic peptide derived from human ATG4C
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4008R-Cy3)?
Cy3 on bs-4008R-Cy3: excitation / emission 512,550nm/570,615nm.