ATG9A Polyclonal Antibody

Lane 1: rat brain lysates Lane 2: rat kidney lysates probed with Anti ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 94kD. Observed band size:94kD.

$Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

$Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n$

Formalin-fixed and paraffin embedded rat stomach labeled with Rabbit Anti-ATG9A Polyclonal Antibody, Unconjugated (bs-4010R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Predicted Reactivity

Human
Mouse
Rat
Dog
Cow
Pig
Rabbit

Overview
Catalog #	bs-4010R
Product Name	ATG9A Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Predicted Reactivity	Human, Mouse, Rat, Dog, Cow, Pig, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ATG9A
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79065
Swiss Prot	Q7Z3C6
Subcellular location	Cytoplasm, Cell membrane
Synonyms	mATG9; APG9L1; MGD3208; Autophagy-related protein 9A; APG9-like 1; ATG9A
Background	Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200