Histone H4 (Acetyl K8) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4016R-BF750-100uL |
| Product Name | Histone H4 (Acetyl K8) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic acetylpeptide derived from human Histone H4 around acetylation site of K8 |
| Modification Site | K8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 121504 |
| Subcellular location | Nucleus |
| Synonyms | Histone H4 Acetyl K8; Histone H4 Acetyl Lys8; Acetyl-Histone H4 Lys8; H4F2; H4FN; HIST1H4; HIST2H4; HISTH4H4; methyl histone H4; histone H4; H4_HUMAN. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
Frequently asked questions
Which applications is Histone H4 (Acetyl K8) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4016R-BF750) validated for, and at what dilution?
bs-4016R-BF750 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Histone H4 (Acetyl K8) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4016R-BF750) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Pig, Horse, Rabbit.
How should bs-4016R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Histone H4 (Acetyl K8) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-4016R-BF750)?
KLH conjugated synthetic acetylpeptide derived from human Histone H4 around acetylation site of K8
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-4016R-BF750)?
AbBy Fluor-750 on bs-4016R-BF750: excitation / emission 749nm/775nm.