Thromboxane synthase Polyclonal Antibody

$Antigen: bs-4019P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n$

Antigen: bs-4019P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-295G-HRP) at 1: 5000; \\nTMB staining; \\nRead the data in MicroplateReader by 450nm. \\n

$Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP5A1\/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP5A1\/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

A549 cell lysates probed with Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: U937 cell lysates; Lane 2: Mouse spleen lysates probed with Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Pig
Horse

Overview
Catalog #	bs-4019R
Product Name	Thromboxane synthase Polyclonal Antibody
Applications	WB, ELISA, IHC-P
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Thromboxane synthase
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6916
Swiss Prot	P24557
Subcellular location	Cytoplasm
Synonyms	TS; TXS; CYP5; THAS; TXAS; CYP5A1; GHOSAL; BDPLT14; Thromboxane-A synthase; TXA synthase; Cytochrome P450 5A1; TBXAS1
Background	Thromboxane Synthase is a useful marker for the detection of native thromboxane synthase in smears, isolated cells, human tissue sections, and for affinity purification of the enzyme. In combination with the markers 27E10, RM 3/1 and 25F9, anti Thromboxane Synthase enables a more precise characterization of inflammatory processes in injured tissues, or in vitro cell-cell interaction studies.Distribution of thromboxane synthase in human tissues: Thromboxane synthase is predominantly produced by macrophages or antigen presenting cells of the myelo-monocytic lineage as shown below. Endothelial cells of placenta and epithelial cells in tonsils and bronchi also express this enzyme.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400