phospho-PRKAR2A (Ser99) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4022R-APC-100uL |
| Product Name | phospho-PRKAR2A (Ser99) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser97 in Rat. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96 |
| Modification Site | Ser96 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5576 |
| Swiss Prot | P13861 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A |
| Background | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PRKAR2A (Ser99) Polyclonal Antibody, APC Conjugated (bs-4022R-APC) validated for, and at what dilution?
bs-4022R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PRKAR2A (Ser99) Polyclonal Antibody, APC Conjugated (bs-4022R-APC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-4022R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PRKAR2A (Ser99) Polyclonal Antibody, APC Conjugated (bs-4022R-APC)?
KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96
What are the excitation and emission wavelengths of the APC conjugate (bs-4022R-APC)?
APC on bs-4022R-APC: excitation / emission 650nm/660nm.