Pancreatic Amylase Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4030R-Cy5.5-100uL |
| Product Name | Pancreatic Amylase Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect both AMY1A and AMY2A. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pancreatic Amylase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 279 |
| Swiss Prot | P04746 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | PA; AMY2; Pancreatic alpha-amylase; 1,4-alpha-D-glucan glucanohydrolase; AMY2A |
| Background | Pancreatic amylase is a digestive enzyme secreted by the pancreas. It consists of a single polypeptide chain with a molecular weight of 54 kDa.Pancreatic amylase and salivary amylase are the products of separate genes and, although they share 98% amino acid sequence homology, they differ in molecular size, isoelectric point and antigenic properties. Measurement of amylase concentrations in serum is a diagnostic test for various pancreatic disorders (especially acute and relapsing pancreatitis). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Pancreatic Amylase Polyclonal Antibody, Cy5.5 Conjugated (BS-4030R-CY5-5) validated for, and at what dilution?
BS-4030R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Pancreatic Amylase Polyclonal Antibody, Cy5.5 Conjugated (BS-4030R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should BS-4030R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pancreatic Amylase Polyclonal Antibody, Cy5.5 Conjugated (BS-4030R-CY5-5)?
KLH conjugated synthetic peptide derived from human Pancreatic Amylase
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4030R-CY5-5)?
Cy5.5 on BS-4030R-CY5-5: excitation / emission 675nm/694nm.