PDHA1(Ser293) Polyclonal Antibody

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Rat Muscle tissue lysates probed with phospho-PDHA1 (Ser293) Polyclonal Antibody, Unconjugated (bs-4036R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Muscle tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Liver tissue lysates; Lane 5: Rat Muscle tissue lysates probed with phospho-PDHA1 (Ser293) Polyclonal Antibody, Unconjugated (bs-4036R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-4036R
Product Name	PDHA1(Ser293) Polyclonal Antibody
Applications	WB, ELISA
Specificity	This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology.
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293
Modification Site	Ser293
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5160
Swiss Prot	P08559
Subcellular location	Cytoplasm
Synonyms	PDHA; PDHAD; PHE1A; PDHCE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1
Background	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000

Applications

Reactivity

Predicted Reactivity

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