phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4036R-Cy3 |
| Product Name | phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Specificity | This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293 |
| Modification Site | Ser293 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5160 |
| Swiss Prot | P08559 |
| Subcellular location | Cytoplasm |
| Synonyms | PDHA; PDHAD; PHE1A; PDHCE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1 |
| Background | The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated (bs-4036R-Cy3) validated for, and at what dilution?
bs-4036R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated (bs-4036R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-4036R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated (bs-4036R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4036R-Cy3)?
Cy3 on bs-4036R-Cy3: excitation / emission 512,550nm/570,615nm.