phospho-PPAR alpha (Ser12) Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4055R-APC-Cy5.5-100uL |
| Product Name | phospho-PPAR alpha (Ser12) Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12 |
| Modification Site | Ser12 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5465 |
| Swiss Prot | Q07869 |
| Subcellular location | Nucleus |
| Synonyms | PPAR; NR1C1; hPPAR; PPARalpha; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; PPARA |
| Background | Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-PPAR alpha (Ser12) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4055R-APC-CY5-5) validated for, and at what dilution?
BS-4055R-APC-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-PPAR alpha (Ser12) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4055R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, GuineaPig.
How should BS-4055R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-PPAR alpha (Ser12) Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-4055R-APC-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-4055R-APC-CY5-5)?
APC-Cy5.5 on BS-4055R-APC-CY5-5: excitation / emission 650nm/694nm.