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phospho-MYL9 (Thr19) Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-4060R-PE-100uL
Product Name phospho-MYL9 (Thr19) Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human MYL9 around the phosphorylation site of Thr19
Modification Site Thr19
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10398
Swiss Prot P24844
Subcellular location Cytoplasm
Synonyms LC2; MLC2; MRLC1; MYRL2; MLC-2C; Myosin regulatory light polypeptide 9; 2 kDa myosin light chain; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1; MYL9
Background Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is phospho-MYL9 (Thr19) Polyclonal Antibody, PE Conjugated (bs-4060R-PE) validated for, and at what dilution?

bs-4060R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does phospho-MYL9 (Thr19) Polyclonal Antibody, PE Conjugated (bs-4060R-PE) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-4060R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise phospho-MYL9 (Thr19) Polyclonal Antibody, PE Conjugated (bs-4060R-PE)?

KLH conjugated synthetic phosphopeptide derived from human MYL9 around the phosphorylation site of Thr19

What are the excitation and emission wavelengths of the PE conjugate (bs-4060R-PE)?

PE on bs-4060R-PE: excitation / emission 496,564nm/578nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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