Glycerol kinase Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4062R-Cy5.5-100uL |
| Product Name | Glycerol kinase Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody will detect GK1 and GK2 due to high sequence similarity. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Glycerol kinase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2710, 2712 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ATP glycerol 3 phosphotransferase; GK; GK1; GK2; GKD; Glycerokinase; Glycerol kinase; Glycerol kinase deficiency; ATP:glycerol 3-phosphotransferase; GLPK_HUMAN. |
| Background | Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors; UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Glycerol kinase Polyclonal Antibody, Cy5.5 Conjugated (BS-4062R-CY5-5) validated for, and at what dilution?
BS-4062R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glycerol kinase Polyclonal Antibody, Cy5.5 Conjugated (BS-4062R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Horse, Rabbit.
How should BS-4062R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glycerol kinase Polyclonal Antibody, Cy5.5 Conjugated (BS-4062R-CY5-5)?
KLH conjugated synthetic peptide derived from human Glycerol kinase 1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4062R-CY5-5)?
Cy5.5 on BS-4062R-CY5-5: excitation / emission 675nm/694nm.