phospho-E2F1 (Ser364) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4076R-Cy7 |
| Product Name | phospho-E2F1 (Ser364) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364 |
| Modification Site | Ser364 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1869 |
| Swiss Prot | Q01094 |
| Subcellular location | Nucleus |
| Synonyms | RBP3; E2F-1; RBAP1; RBBP3; Transcription factor E2F1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1 |
| Background | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-E2F1 (Ser364) Polyclonal Antibody, Cy7 Conjugated (bs-4076R-Cy7) validated for, and at what dilution?
bs-4076R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-E2F1 (Ser364) Polyclonal Antibody, Cy7 Conjugated (bs-4076R-Cy7) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-4076R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-E2F1 (Ser364) Polyclonal Antibody, Cy7 Conjugated (bs-4076R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser364
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4076R-Cy7)?
Cy7 on bs-4076R-Cy7: excitation / emission 743nm/767nm.