phospho-IRAK4 (Thr345) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-4080R-RBITC |
| Product Name | phospho-IRAK4 (Thr345) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345 |
| Modification Site | Thr345 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51135 |
| Swiss Prot | Q9NWZ3 |
| Subcellular location | Cytoplasm |
| Synonyms | IPD1; REN64; IRAK-4; NY-REN-64; Interleukin-1 receptor-associated kinase 4; Renal carcinoma antigen NY-REN-64; IRAK4 |
| Background | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Application Dilution | |
| WB | |
| IF(IHC-P) | |
Frequently asked questions
Which applications is phospho-IRAK4 (Thr345) Polyclonal Antibody, RBITC Conjugated (bs-4080R-RBITC) validated for, and at what dilution?
bs-4080R-RBITC is validated for WB, IF(IHC-P).
Which species does phospho-IRAK4 (Thr345) Polyclonal Antibody, RBITC Conjugated (bs-4080R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-4080R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-IRAK4 (Thr345) Polyclonal Antibody, RBITC Conjugated (bs-4080R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345