Help

NSE Polyclonal Antibody, PE-Cy5 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bs-41181R-PE-Cy5-100uL
Product Name NSE Polyclonal Antibody, PE-Cy5 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation PE-Cy5
Host Rabbit
Source Recombinant human NSE
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2026
Swiss Prot P09104
Subcellular location Cytoplasm, Cell membrane
Synonyms Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enolase; NSE; ENOG_HUMAN; Gamma-enolase; 2-phospho-D-glycerate hydro-lyase; enolase 2, gamma, neuronal.
Background Gamma-enolase, also known as enolase 2 (ENO2) or neuron specific enolase (NSE), is an enzyme that in humans is encoded by the ENO2 gene. Gamma-enolase is a phosphopyruvate hydratase. Gamma-enolase is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is NSE Polyclonal Antibody, PE-Cy5 Conjugated (bs-41181R-PE-Cy5) validated for, and at what dilution?

bs-41181R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does NSE Polyclonal Antibody, PE-Cy5 Conjugated (bs-41181R-PE-Cy5) react with?

Tested reactivity: Human.

How should bs-41181R-PE-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise NSE Polyclonal Antibody, PE-Cy5 Conjugated (bs-41181R-PE-Cy5)?

Recombinant human NSE

What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-41181R-PE-Cy5)?

PE-Cy5 on bs-41181R-PE-Cy5: excitation / emission 488nm/670nm.