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Laminin alpha 1 Polyclonal Antibody

Lane 1: Recombinant human Laminin Alpha-1 protein probed with Laminin alpha 1 Polyclonal Antibody, Unconjugated (bs-41199R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Recombinant human Laminin Alpha-1 protein probed with Laminin alpha 1 Polyclonal Antibody, Unconjugated (bs-41199R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
4% Paraformaldehyde-fixed JAR (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Laminin alpha 1) polyclonal Antibody, unconjugated (bs-41199R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
4% Paraformaldehyde-fixed JAR (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Laminin alpha 1) polyclonal Antibody, unconjugated (bs-41199R) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The JAR (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Laminin alpha 1 antibody (bs-41199R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The JAR (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-Laminin alpha 1 antibody (bs-41199R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bs-41199R
Product Name Laminin alpha 1 Polyclonal Antibody
Applications
WB
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Laminin alpha 1
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 284217
Swiss Prot P25391
Subcellular location Secreted, Cell membrane, Extracellular matrix
Synonyms LAMA; LAMA1; Laminin A chain; Laminin subunit alpha-1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-laminin subunit alpha; S-LAM alpha; Laminin subunit alpha-1; LAMA1_HUMAN; Laminin-1 subunit alpha; Laminin-3 subunit alpha.
Application Dilution
WB =1:500-2000

Frequently asked questions

Which applications is Laminin alpha 1 Polyclonal Antibody (bs-41199R) validated for, and at what dilution?

bs-41199R is validated for WB (WB=1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Laminin alpha 1 Polyclonal Antibody (bs-41199R) react with?

Tested reactivity: Human.

How should bs-41199R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Laminin alpha 1 Polyclonal Antibody (bs-41199R)?

KLH conjugated synthetic peptide derived from human Laminin alpha 1

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