phospho-MEK3 (Thr222) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4123R-BF680-100uL |
| Product Name | phospho-MEK3 (Thr222) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of Thr222 |
| Modification Site | Thr222 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5606 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Dual specicity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; map2k3; MAPK ERK kinase 3; MAPK/ERK kinase 3; MAPKK 3; MAPKK3; MEK 3; MEK3; Mitogen activated protein kinase kinase 3; MKK3; MP2K3_HUMAN; PRKMK3. |
| Background | Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MEK3 (Thr222) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4123R-BF680) validated for, and at what dilution?
bs-4123R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MEK3 (Thr222) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4123R-BF680) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Horse, Chicken.
How should bs-4123R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MEK3 (Thr222) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-4123R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of Thr222
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-4123R-BF680)?
AbBy Fluor-680 on bs-4123R-BF680: excitation / emission 679nm/702nm.