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PAP2B Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
Overview
Catalog # bs-4127R-Cy7
Product Name PAP2B Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Dog, Cow, Pig, Horse
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PAP2B
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 8613
Swiss Prot O14495
Subcellular location Cytoplasm, Cell membrane
Synonyms LPP3; VCIP; Dri42; PAP2B; Lipid phosphate phosphohydrolase 3; PAP2-beta; Phosphatidate phosphohydrolase type 2b; Phosphatidic acid phosphatase 2b; PAP-2b; Vascular endothelial growth factor and type I collagen-inducible protein; PPAP2B
Background Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PAP2B Polyclonal Antibody, Cy7 Conjugated (bs-4127R-Cy7) validated for, and at what dilution?

bs-4127R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PAP2B Polyclonal Antibody, Cy7 Conjugated (bs-4127R-Cy7) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse.

How should bs-4127R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PAP2B Polyclonal Antibody, Cy7 Conjugated (bs-4127R-Cy7)?

KLH conjugated synthetic peptide derived from human PAP2B

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-4127R-Cy7)?

Cy7 on bs-4127R-Cy7: excitation / emission 743nm/767nm.