MAPK4 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4131R-Biotin |
| Product Name | MAPK4 Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ERK4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 5596 |
| Swiss Prot | P31152 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK; Mitogen-activated protein kinase 4; MAP kinase 4; MAPK 4; Extracellular signal-regulated kinase 4; MAP kinase isoform p63; MAPK4 |
| Background | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is MAPK4 Polyclonal Antibody, Biotin Conjugated (bs-4131R-Biotin) validated for, and at what dilution?
bs-4131R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAPK4 Polyclonal Antibody, Biotin Conjugated (bs-4131R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should bs-4131R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAPK4 Polyclonal Antibody, Biotin Conjugated (bs-4131R-Biotin)?
KLH conjugated synthetic peptide derived from human ERK4