MAPK4 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4131R-PE-Cy5.5-100uL |
| Product Name | MAPK4 Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ERK4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5596 |
| Swiss Prot | P31152 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK; Mitogen-activated protein kinase 4; MAP kinase 4; MAPK 4; Extracellular signal-regulated kinase 4; MAP kinase isoform p63; MAPK4 |
| Background | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is MAPK4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-4131R-PE-CY5-5) validated for, and at what dilution?
BS-4131R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAPK4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-4131R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should BS-4131R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAPK4 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-4131R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from human ERK4
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-4131R-PE-CY5-5)?
PE-Cy5.5 on BS-4131R-PE-CY5-5: excitation / emission 488nm/694nm.