CH25H, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-41322R-Biotin-100uL |
| Product Name | CH25H, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | Recombinant human CH25H protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 9023 |
| Swiss Prot | O95992 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | C25H; CH25H; Cholesterol 25 hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN. |
| Background | CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. |
| Application Dilution | |
| WB | =1:500-2000 |
| IHC-P | IHC-P=1:400-800 |
| IHC-F | IHC-F=1:400-800 |
Frequently asked questions
Which applications is CH25H, Biotin Conjugated (bs-41322R-Biotin) validated for, and at what dilution?
bs-41322R-Biotin is validated for WB (WB=1:500-2000); IHC-P (IHC-P=1:400-800); IHC-F (IHC-F=1:400-800). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CH25H, Biotin Conjugated (bs-41322R-Biotin) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-41322R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CH25H, Biotin Conjugated (bs-41322R-Biotin)?
Recombinant human CH25H protein