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MAPK11 Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
  • Dog
  • Horse
Overview
Catalog # bs-4138R-PerCP-Cy5.5-100uL
Product Name MAPK11 Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Rat
Predicted Reactivity Mouse, Dog, Horse
Specifications
Conjugation PerCP-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MAPK11
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5600
Swiss Prot Q15759
Subcellular location Cytoplasm, Nucleus
Synonyms P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2; Mitogen-activated protein kinase 11; MAP kinase 11; MAPK 11; Mitogen-activated protein kinase p38 beta; MAP kinase p38 beta; Stress-activated protein kinase 2b; MAPK11
Background The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. MAPK11 is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is MAPK11 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-4138R-PERCP-CY5-5) validated for, and at what dilution?

BS-4138R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does MAPK11 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-4138R-PERCP-CY5-5) react with?

Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Horse.

How should BS-4138R-PERCP-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise MAPK11 Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-4138R-PERCP-CY5-5)?

KLH conjugated synthetic peptide derived from human MAPK11

What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-4138R-PERCP-CY5-5)?

PerCP-Cy5.5 on BS-4138R-PERCP-CY5-5: excitation / emission 482nm/694nm.

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