Glutamine synthetase Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4143R-BF555-100uL |
| Product Name | Glutamine synthetase Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Glutamine syntase/Gl Syn |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2752 |
| Swiss Prot | P15104 |
| Subcellular location | Cytoplasm |
| Synonyms | GS; GLNS; PIG43; PIG59; Glutamine synthetase; Glutamate decarboxylase; Glutamate--ammonia ligase; GLUL |
| Background | This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Glutamine synthetase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4143R-BF555) validated for, and at what dilution?
bs-4143R-BF555 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glutamine synthetase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4143R-BF555) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig.
How should bs-4143R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glutamine synthetase Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-4143R-BF555)?
KLH conjugated synthetic peptide derived from human Glutamine syntase/Gl Syn
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-4143R-BF555)?
AbBy Fluor-555 on bs-4143R-BF555: excitation / emission 553nm/568nm.