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Glutamine synthetase Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Cow
  • Sheep
  • Pig
Overview
Catalog # bs-4143R-PE-100uL
Product Name Glutamine synthetase Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Cow, Sheep, Pig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Glutamine syntase/Gl Syn
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2752
Swiss Prot P15104
Subcellular location Cytoplasm
Synonyms GS; GLNS; PIG43; PIG59; Glutamine synthetase; Glutamate decarboxylase; Glutamate--ammonia ligase; GLUL
Background This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Glutamine synthetase Polyclonal Antibody, PE Conjugated (bs-4143R-PE) validated for, and at what dilution?

bs-4143R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Glutamine synthetase Polyclonal Antibody, PE Conjugated (bs-4143R-PE) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig.

How should bs-4143R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Glutamine synthetase Polyclonal Antibody, PE Conjugated (bs-4143R-PE)?

KLH conjugated synthetic peptide derived from human Glutamine syntase/Gl Syn

What are the excitation and emission wavelengths of the PE conjugate (bs-4143R-PE)?

PE on bs-4143R-PE: excitation / emission 496,564nm/578nm.

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