PKMYT1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4148R-PE-Cy5-100uL |
| Product Name | PKMYT1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Horse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKMYT1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9088 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | cdc2 inhibitory kinase; Membrane associated tyrosine and threonine specic cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specic cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; DKFZp547K1610; FLJ20093. |
| Background | PKMYT1 is a member of the serine/threonine protein kinase family. It preferentially phosphorylates and inactivates cell division cycle 2 protein (cdc2), and thus acts as a negative regulator of entry into mitosis (G2 to M transition). It mediates phosphorylation of cdc2 predominantly on 'Thr-14' and is also involved in Golgi fragmentation. It may be involved in phosphorylation of cdc2 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. It may be a downstream target of Notch signaling pathway during eye development. PKMYT1 is negatively regulated by hyperphosphorylation during mitosis. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is PKMYT1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-4148R-PE-Cy5) validated for, and at what dilution?
bs-4148R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4148R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKMYT1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-4148R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human PKMYT1
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-4148R-PE-Cy5)?
PE-Cy5 on bs-4148R-PE-Cy5: excitation / emission 488nm/670nm.