PTPN7 Polyclonal Antibody, Cy3 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4155R-Cy3 |
| Product Name | PTPN7 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PTPN7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5778 |
| Subcellular location | Cytoplasm |
| Synonyms | HePTP; BPTP 4; BPTP4; Dual specicity phosphatase 1; Hematopoietic protein tyrosine phosphatase; LC PTP; LCPTP; LPTP; Protein tyrosine phoshatase non receptor type stress induced; Protein tyrosine phoshatase nonreceptor type stress induced; Protein tyrosine phosphatase LC PTP; Protein tyrosine phosphatase non receptor type 7; PTPN 7; PTPNI; Tyrosine protein phosphatase non receptor type 7; PTN7_HUMAN. |
| Background | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The non-catalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PTPN7 Polyclonal Antibody, Cy3 Conjugated (bs-4155R-Cy3) validated for, and at what dilution?
bs-4155R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-4155R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTPN7 Polyclonal Antibody, Cy3 Conjugated (bs-4155R-Cy3)?
KLH conjugated synthetic peptide derived from human PTPN7
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4155R-Cy3)?
Cy3 on bs-4155R-Cy3: excitation / emission 512,550nm/570,615nm.