TNIK Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4168R-BF350-100uL |
| Product Name | TNIK Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TNIK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23043 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Traf2 and NCK interacting kinase; 1500031A17RIK; 4831440I19RIK; AI451411; C530008O15Rik; C630040K21RIK; KIAA0551; MGC189819; MGC189859; RGD1561817; TNIK; TNIK_HUMAN; TRAF2 and NCK-interacting protein kinase. |
| Background | TNIK is a MSN protein kinase that interacts with both TNF receptor-associated factor 2 (TRAF2) and the adapter protein NCK. The protein has been shown to activate the c-Jun N-terminal kinase pathway when over expressed in Phoenix-A cells. TNIK has been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton. Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder and skin. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TNIK Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4168R-BF350) validated for, and at what dilution?
bs-4168R-BF350 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TNIK Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4168R-BF350) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-4168R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TNIK Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-4168R-BF350)?
KLH conjugated synthetic peptide derived from human TNIK
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-4168R-BF350)?
AbBy Fluor-350 on bs-4168R-BF350: excitation / emission 346nm/442nm.