PTK9 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4169R-Cy5-100uL |
| Product Name | PTK9 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TWF1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5756 |
| Swiss Prot | Q12792 |
| Subcellular location | Cytoplasm |
| Synonyms | A6; A6 protein tyrosine kinase; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF 1; TWF1; Twinfilin 1; Twinfilin actin binding protein, homolog 1; Twinfilin; Twinfilin, actin-binding protein, homolog 1 Drosophila; Twinfilin, Drosophila, homolog of, 1; TWF1_HUMAN; Twinfilin-1. |
| Background | PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PTK9 Polyclonal Antibody, Cy5 Conjugated (bs-4169R-Cy5) validated for, and at what dilution?
bs-4169R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTK9 Polyclonal Antibody, Cy5 Conjugated (bs-4169R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.
How should bs-4169R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTK9 Polyclonal Antibody, Cy5 Conjugated (bs-4169R-Cy5)?
KLH conjugated synthetic peptide derived from human TWF1
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-4169R-Cy5)?
Cy5 on bs-4169R-Cy5: excitation / emission 625,650nm/670nm.