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CD68 Polyclonal Antibody, RBITC Conjugated

Applications

  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-42013R-RBITC-100uL
Product Name CD68 Polyclonal Antibody, RBITC Conjugated
Applications
FCM
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation RBITC
Host Rabbit
Source Recombinant mouse CD68 protein
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 12514
Swiss Prot P31996
Subcellular location Cytoplasm, Cell membrane
Background This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is CD68 Polyclonal Antibody, RBITC Conjugated (bs-42013R-RBITC) validated for, and at what dilution?

bs-42013R-RBITC is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD68 Polyclonal Antibody, RBITC Conjugated (bs-42013R-RBITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-42013R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD68 Polyclonal Antibody, RBITC Conjugated (bs-42013R-RBITC)?

Recombinant mouse CD68 protein

What are the excitation and emission wavelengths of the RBITC conjugate (bs-42013R-RBITC)?

RBITC on bs-42013R-RBITC: excitation / emission 540nm/625nm.

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US only. One sample per paid order, subject to review. Backorders may ship separately. Confirm Free at checkout. Check application and species suitability; same target does not guarantee equivalent performance. For research use only.

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