FIH1/HIF1AN Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-4201R-Cy5.5-100uL |
| Product Name | FIH1/HIF1AN Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FIH1/HIF1AN |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55662 |
| Subcellular location | Nucleus |
| Synonyms | Hypoxia inducible factor asparagine hydroxylase; Factor inhibiting H1; FIH 1; FIH-1; FIH1; Hypoxia inducible factor 1 alpha inhibitor; Hypoxia inducible factor 1 alpha subunit inhibitor; Peptide aspartate beta dioxygenase; H1AN; DKFZp762F1811; FIH1; H1AN; FLJ20615; FLJ22027; H1N_HUMAN. |
| Background | HIF1AN (Hypoxia inducible factor 1 alpha inhibitor) is a corepressor that interacts with hypoxia inducible factor 1 (HIF1) alpha and the von Hippel Lindau tumor suppressor protein to mediate repression of HIF1 transcriptional activity. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FIH1/HIF1AN Polyclonal Antibody, Cy5.5 Conjugated (BS-4201R-CY5-5) validated for, and at what dilution?
BS-4201R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-4201R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FIH1/HIF1AN Polyclonal Antibody, Cy5.5 Conjugated (BS-4201R-CY5-5)?
KLH conjugated synthetic peptide derived from human FIH1/HIF1AN
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-4201R-CY5-5)?
Cy5.5 on BS-4201R-CY5-5: excitation / emission 675nm/694nm.