Cullin 1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-4208R-Cy3-100uL |
| Product Name | Cullin 1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Cullin 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8454 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cullin-1; CUL 1; CUL1; Cullin1; MGC149834; MGC149835. |
| Background | Cullin 1 may be involved in the degradation of positive cell cycle regulators (for example G1 cyclins in yeast). Human cullin 1 forms a complex with Skp1 and the F box protein Skp2, which is thought to play a role in the ubiquitination of G1 regulatory proteins, as its homolog does in yeast. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Cullin 1 Polyclonal Antibody, Cy3 Conjugated (bs-4208R-Cy3) validated for, and at what dilution?
bs-4208R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cullin 1 Polyclonal Antibody, Cy3 Conjugated (bs-4208R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Rabbit.
How should bs-4208R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cullin 1 Polyclonal Antibody, Cy3 Conjugated (bs-4208R-Cy3)?
KLH conjugated synthetic peptide derived from human Cullin 1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-4208R-Cy3)?
Cy3 on bs-4208R-Cy3: excitation / emission 512,550nm/570,615nm.